The Laboratory of Tissue Regeneration

Lahey Hospital & Medical Center’s Laboratory of Tissue Regeneration focuses on the most significant problems in reconstructive surgery, motivated by many of our cancer and trauma patients. Whether developing new strategies to treat serious wounds, coming up with innovative ways of connecting small blood vessels or studying methods to re-grow nervous tissue, the Laboratory of Tissue Regeneration staff is dedicated to improving patient outcomes. These efforts provide the foundation for further advancement in healing technologies, targeting patients with major reconstructive needs.

As one of the leading laboratories in wound healing research, the Laboratory of Tissue Regeneration uses a unique mouse model to study the various aspects of the wound healing process, finding new and better ways to improve the quality and time of healing. With the growth of complicated surgeries, wound healing research is an essential aspect of reconstructive surgery.

The laboratory is also working towards improving the efficiency, safety and reliability of microvascular anastomosis: a technically demanding and time consuming surgery to reconnect the blood vessels to allow transfer of tissues for coverage of large defects. The goal of Lahey’s Laboratory of Tissue Regeneration is to benefit cancer, trauma and transplant patients by making such free tissue transfer a routine surgery.

In addition, the lab is working in collaboration with another lab to develop a new method of gene transfer in neuronal tissue that they expect will enhance peripheral nerve regeneration. Advancements in this research will help patients regain function and sensation in their bodies after major cancer and trauma procedures.

Lifei Guo, MD, PhD, FACS, Director    

Selected Publications

  • Guo L, Yu Q-C, Fuchs E. Targeting expression of keratinocyte growth factor to keratinocytes elicits striking changes in epithelial differentiation in transgenic mice. The EMBO Journal. 1993;12(3):973-986.
  • Guo L, Degenstein L, Dowling J, Yu Q-C, Wollmann R, Perman B, Fuchs E. Gene targeting of BPAG1: abnormalities in mechanical strength and cell migration in stratified epithelia and neurologic degeneration. Cell. 1995;81:223-243.
  • Guo L, Degenstein L, Fuchs E. Keratinocyte growth factor is required for hair development but not for wound healing. Genes & Development. 1996;10:165-175.
  • Guo L, Kubat NJ, Isenberg RA. Pulsed radio frequency energy (PRFE) use in human medical applications. Electromagnetic Biology and Medicine. 2011;30(1):21-45.
  • Li Q, Kao HK, Matros E, Peng C, Murphy GF, Guo L. Pulsed radio frequency energy (PRFE) accelerates wound healing in diabetic mice. Plastic and Reconstructive Surgery. 2011;127(6):2255-62.
  • Peng C, Chen B, Kao HK, Murphy GF, Orgill DP, Guo L. Lack of FGF-7 further delays cutaneous wound healing in diabetic mice. Plastic and Reconstructive Surgery. 2011;128(6):673-84e.
  • Kao HK, Chen B, Murphy G, Li Q, Orgill DP, Guo L. Peripheral blood fibrocytes: enhancement of wound healing by cell proliferation, re-epithelialization, contraction, and angiogenesis. Annals of Surgery. 2011;254(6):1066-74.
  • Guo L, Kubat NJ, Nelson TR, Isenberg, RA. Meta-analysis of clinical efficacy of pulsed radio frequency energy treatment. Annals of Surgery. 2012;255(3):457-67.
  • Cheriyan T, Guo L, Orgill DP, Padera RF, Schmid TM, Spector M. Lubricin in human breast tissue expander capsules. Journal of Biomedical Materials Research. 2012;100B(7):1961-1969.
  • Kao HK, Li Q, Flynn B, Qiao X, Ruberti JW, Murphy GF, Guo L. Collagen synthesis modulated in wounds treated by pulsed radiofrequency energy. Plastic and Reconstructive Surgery. 2013;131(4):490-8e.
  • Cheriyan T, Kao HK, Qiao X, Guo L. Low harvest pressure enhances autologous fat graft viability. Plastic and Reconstructive Surgery (In press).